The Space Cats come from a planet called Trygliceride-7, ruled by a being named D.O.R.C. (short for Disembodied Omnipotent Ruler of Cats) which can be described as a disembodied, bespectacled, human head with a funny voice. The Space Cats station themselves on Earth in an underground base with a garbage can as its only access. A live-action segment is shown, where various Space Cats are seen on duty.
D.O.R.C. describes each mission to the team's leader Captain Catgut. The animated segment is then shown where Captain Catgut sends the team of Tom, Scratch and Sniff out to work. At the end of each episode, the group gives the viewers a moral, then wrap up the episode vocalizing the Charge music and shouting out their team name. This was spoofed, when one Space Cat says the moral is for kids to stop watching TV and go read a book, the other two angrily remark that if that is taken seriously it would result in their own cancellation!