First match of All-Japan National Tournament: Rokkaku vs. Higa. Ojii explains the Shukuchi Method, and Ryoma comes back from America!
Seigaku's first round's matches start with Higa Middle School. Ryoma faces Kei Tanishi, and shows his souvenir from America - the Cool Drive, as well as Drive C, a less powerful version of the Cool Drive!
Fuji and Kawamura play together in the Doubles 2 match. During the match, Higa's coach signals his player to attack Ryuzaki-sensei with the ball. Fuji and Kawamura get angry at this and start playing at full force. However, it's not that easy because of the Shukuchi Method. Fuji uses his Fourth Counter: Kagerou Zutsumi, a.k.a. Dragonfly Illusion.
Kikumaru plays Singles 2 against Kai from Higa. During the game, it is shown how hard Kikumaru had trained to raise his stamina. His lateral movement has improved so much that it looks like there are two Kikumarus. This motion is called Seal Step, and it looks as if he has a doppleganger.
Rikkai Dai's Yagyuu meets Kaidoh and asks him what he would do without his Snake. Kaido then asks Inui to let him play Doubles by himself and see how far he could go without his signature move.
Tezuka plays against Higa Chuu's Captain, Eishirou Kite. Kite shows that he would do anything to win, even throwing small pebbles at Tezuka. Tezuka shows everyone his true form using Hyaku Ren Jitoku no Kiwami - The Pinnacle of Hard Work.
In between matches, Seigaku is now a volleyball team and plays with Rokkaku before training for the next match.
Hyotei reminisces about their loss at the Kantou tournament and strive to win the quarterfinals against Seigaku.
The first match in the quarterfinals is Oshitari vs Momoshiro! Momoshiro in the Singles 3 Spot shows off his new style of play.
Doubles 2 match of the quarterfinals: Inui-Kaido pair vs Mukahi-Hiyoshi pair. In a try for a short match against Inui and Kaido, Hiyoshi and Mukahi play with all they've got from the beginning. Also, Kaido and Inui show two new moves!
Tezuka vs Kabaji in Singles 2 of the quarterfinals. An untold history about Tezuka in the Kyûshu Region before his rehabilitation is shown. In the present time, can Tezuka stand up to Kabaji's imitation of himself?
A duel of the Golden Pairs: Kikumaru-Oishi vs Shishido-Ootori. Ootori's improved Neo Scud Serve & the Pile-Mirage formation puts Seigaku's Golden Pair to the test! But the Golden Pair achieves a new state: Synchro! However, the win goes to Hyotei as Kikumaru "prevents" Oishi from hitting the ball further and causing more injury to his wrist.
In Singles 1, Ryoma faces Atobe to see who advances to the semi-finals of the national tournament. Ryoma uses the State of Self-Actualization, but it is useless against Atobe and his new move, "Koori no Sekai". But Ryoma counters Koori no Sekai with an incomplete version of his father's "Zone" technique, which is the same as the "Tezuka Zone". Eventually, the two go into what seems like a never-ending tie-break.