Consisting of low-budget films, bookended by in-studio or on-location comedy skits featuring the show's hosts. In addition to skits, the hosts would also provide sardonic comments about the featured film(s), and observations on various Hollywood- and/or New York City-area clubs and attractions (when the series was shooting out of studio). Including commercials, the program typically ran from 11 p.m. to 5 a.m.
The movies aired ranged from cult classics, to B movies, to other fare not appearing on television frequently. Up All Night would regularly show sexploitation films, with the explicit content edited out.